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Distal-less homeobox 3 (DLX3) is an important homeobox gene that controls the differentiation of the osteoblast lineage. Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine as an excellent cell source for bone tissue engineering and regeneration. However, the role of DLX3 in the proliferation and osteogenic differentiation of MSCs is still unclear. In this study, we investigated the role of DLX3 in the proliferation and osteogenic differentiation of human MSCs. Real-time RT-PCR analysis showed that the expression of DLX3 was significantly increased in human MSCs after osteogenic differentiation. Transfection with the DLX3 plasmid enhanced the expression of DLX3 and osteogenic differentiation, and transfection with the DLX3 shRNA inhibited these processes. Furthermore, we found that the expression level of DLX3 correlated with the proliferation of MSCs in various culture conditions. These results indicate that DLX3 plays an important role in the proliferation and osteogenic differentiation of MSCs, and thus might be a potential target for regenerative medicine.Cytomegalovirus infection: prevention by vaccination.
Although there are reports of successful post-exposure vaccine protection for cytomegalovirus, it is unlikely that any single vaccine will be effective to the majority of healthy people. Vaccines are more likely to be effective against disease by inducing protective cellular immunity and blocking viral replication, rather than by directly preventing infection. Although cytomegalovirus is a common infection of children, immunologic responses to natural infection are poorly characterized and do not predict future risk. Thus, vaccination is more likely to be effective in preventing primary infection than in preventing reinfection or clinical disease. Although there are no licensed vaccines for cytomegalovirus, various vaccine strategies are under evaluation.Cryopreservation of human oocytes and embryos and the effect of thawing on developmental rate: an in vitro study.
In vitro fertilization (IVF) is limited by the decreased number of oocytes available for fertilization. Our aim was to freeze unfertil